Figure 7

Figure 7

Aesculetin recued the immunotoxicity induced by OTA exposure. (A, B) Representative images of control, 25 ng/mL OTA and 25 ng/mL OTA + 20 μg/mL aesculetin-treated Tg(lyz:dsRed)(A) and Tg(mpx:EGFP)(B) transgenic zebrafish larvae at 3 dpf. The white dashed rectangle represents the CHT region and the white arrows represent the neutrophils. Scale bar, 100 μm. (C, D) Statistical analysis of the number of neutrophils in Tg(lyz:dsRed)(C) and Tg(mpx:EGFP)(D) transgenic zebrafish larvae. One-way ANOVA-Dunnett test, *p < 0.05, ***p < 0.001. Error bar represents standard deviation. (E, F) qRT-PCR results showing the expression level of anxa1a(E) and anxa1d(F) in control, 25 ng/mL OTA treated and 25 ng/mL OTA + 20 μg/mL aesculetin treated zebrafish larvae at 3 dpf. One-way ANOVA-Dunnett test, *p < 0.05, **p < 0.01. Error bar represents standard deviation. (G) Survival rate of zebrafish larvae exposed to 0, 25 ng/mL OTA and 25 ng/mL OTA + 20 μg/mL aesculetin at 2 dpf and 3 dpf. One-way ANOVA-Dunnett test, ***p < 0.001. Error bar represents standard deviation. (H) Schematic diagram of OTA-induced immunotoxicity in zebrafish.