OTA impaired the formation of innate immune cells in zebrafish larvae. (A, B) Representative images of control and OTA-treated Tg(lyz:dsRed)(A) and Tg(mpx:EGFP)(B) transgenic zebrafish larvae at 3 dpf. The white dashed rectangle represents the CHT region and the white arrows represent the neutrophils. Scale bar, 100 μm. (C) Representative neutral red staining results of control and OTA-treated zebrafish larvae at 3 dpf. The black arrows represent neutral red labeled macrophages. Scale bar, 100 μm. (D–F) Statistical analysis of the number of neutrophils (A, B) and macrophages (C). One-way ANOVA-Dunnett test, *p < 0.05, ***p < 0.001. Error bar represents standard deviation.
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