FIGURE 2

FIGURE 2

Ocular coloboma and sensory hair cell defects in ntn1a morphant zebrafish. (A) Fusion of the optic fissure was not complete in ntn1a morphant embryos at 56 h post‐fertilisation. The arrow indicates the ocular coloboma. (B) The lateral line neuromasts were visualised in control and ntn1a larvae at 5 days post‐fertilisation (dpf). Anti‐acetylated tubulin (AT, green) detected the hair cell bodies and kinocilia and Alexa Fluor 647 Phalloidin (red) stained the stereociliary hair bundles. The ntn1a neuromasts lacked hair cell bodies, hair bundles and kinocilia. (C) The stereociliary hair bundles of the anterior macula in the inner ear were visualised used Alexa Fluor 647 Phalloidin (white). The hair bundle morphology appeared to be normal in ntn1a at 5 dpf, but the hair bundles were reduced in number. (D) The bar charts show that the numbers of hair bundles per neuromast (n = 9, *p < 0.05) and per anterior macula (n = 5, ***p < 0.001) were significantly reduced in the ntn1a morphant compared with control larvae at 5 dpf. Scale bars = 10 μm.