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Fig. 7 - Supplemental 1 Expression of POST-IT does not cause toxicity in zebrafish. (A) Quantitative analysis shows that embryos injected with POST-IT plasmid exhibit no signs of toxicity. Embryos were injected at one-cell stage, and phenotypes were assessed 24 hr later. n = 3. Data are shown as mean ± s.e.m. (B) Representative images of transgenic zebrafish embryos expressing heat-shock-inducible POST-IT show no obvious toxicity after heat shock. EGFP images confirm robust expression of POST-IT. Healthy embryos at 1 dpf were treated at 39°C for 30 min and imaged at 2 dpf. Scale bar, 500 µm. (C) Quantitative data related to (B) indicate that POST-IT expression following heat treatment does not cause toxicity. n = 3. Data are shown as mean ± s.e.m.