Fig. 3

Fig. 3

Snap25b knockout causes embryonic development defects and decreases swimming activity. A The snap25b gene structure and the schema for CRISPR-Cas9 technology to reduce snap25b expression in zebrafish. B Sanger sequencing confirms the mutations caused by CRIPSR-Cas9 in F0 and F1 generations. Primary sequence was shown. C In situ hybridization of snap25b in wild-type (WT) and snap25b mutant zebrafish. D The expression of snap25b by RT-qPCR in the head of wild-type and snap25b mutant zebrafish. E The representative abnormal phenotype included developmental delay, curled tail, and agenesis of eye. F The summary of survival rate in wild-type and snap25b mutants. G The summary of abnormality rate in wild-type and snap25b mutants. The Danio Vision system recorded the swimming behavior of wild-type and snap25b mutants (H) and analysis of total swimming distance (I). Data are presented as mean ± SD, n = 3 or n = 15–18; ** p < 0.01