Figure 1

Figure 1

The deletion of dhx38 caused inner ear development defects: (A) The different morphological specificities of the inner ears of different genotypes at distinct developmental stages. Scale bar: 40 µm. The black arrow indicates the otic vesicle, the white arrow indicates the otolith, and the red arrow indicates semicircular canal protrusion. (B) Statistical analysis of the otic area and otolith area in the embryos of different genotypes at 42 hpf, 45 hpf, 48 hpf, and 56 hpf. Individual embryos were picked casually from particular types for statistical analysis. The otic and otolith areas were measured using SPOT Advanced software (version 4.6) in the focal plane exhibiting the maximal area [16]. n, the number of checked embryos. n = 40. (C) The percentages of embryos that had semicircular canal protrusions in the sibling, the dhx38^+/− and dhx38^−/− groups at 48 hpf and 56 hpf. The data are presented as the mean ± SD. ns, p > 0.05; *, p < 0.05; ***, p < 0.001.