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Fig. 3 Disrupted expression of circadian clock genes in mir-219-1−/−, mir-219-2−/−, and mir-219-3−/− zebrafish larvae.Expression of mature miR-219-5p (A), mir-219-2 (B), mir-219-3 (C), bmal1a (D), bmal1b (E), clock1a (F), per1b (G), per2 (H), and cry1bb (I) in the mir-219-1−/− mutant larvae (purple). Data are mean ± SD. n = 3 × 3. Expression of mature miR-219-5p (J), mir-219-1 (K), mir-219-3 (L), bmal1a (M), bmal1b (N), clock1a (O), per1b (P), per2 (Q), and cry1bb (R) in the mir-219-2−/− mutant larvae (red). Data are mean ± SD. n = 3 × 3. Expression of mature miR-219-5p (S), mir-219-1 (T), mir-219-2 (U), bmal1a (V), bmal1b (W), clock1a (X), per1b (Y), per2 (Z), and cry1bb (Z’) in the mir-219-3−/− mutant larvae (green). Data are mean ± SD. n = 3 × 3. JTK_CYCLE was performed, P < 0.05 indicates rhythmic expression. Two-way ANOVA with the Tukey post hoc test was conducted, *P < 0.05, **P < 0.01, ***P < 0.001 vs. WT.