Fig.5

Fig.5

Maternal dietary DHA restriction and biosynthesis disruption depletes offspring DHA and increases ARA. Adult wild-type and elovl2^+/− parents were fed either SUFF or DEF for 25–29 weeks prior to spawning. A: DHA and (B) ARA concentrations in 0 hpf embryos and 4 dpf larvae measured by GC/MS. Offspring from elovl2-DEF mothers had similar fatty acid status, regardless of +/+ or −/− genotype. Dots represent individually analyzed offspring (n = 6–12 fish/group). Groups labeled with different letters denote significant differences by 1-way ANOVA with Tukey’s post-hoc test (P < 0.05). C: Representative DHA-PL and ARA-PL species in 4 dpf larvae measured by LC/MS/MS (n = 3 pooled replicates/group; n = 3 larvae per pool). Values are log₂(fold change) relative to the WT-SUFF group mean. DHA-PLs decreased and ARA-PLs increased in offspring from DEF-fed mothers compared to the WT-SUFF group. ARA, arachidonic acid; DHA, docosahexaenoic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PL, phospholipid; PS, phosphatidylserine.