Figure 4.

Figure 4.

Significant effects associated with one-carbon metabolism induced by incorporated C-14 in zebrafish. (a) Alteration of global DNA 5mC methylation level after accumulating no (not detectable, ND), low (10.9 bq mg⁻¹) and high (85.4 bq mg⁻¹) concentrations of C-14 in zebrafish brain. (b) Average velocity and corresponding (c) representative trajectories recorded from zebrafish ingested OBC-14 via trophic transfer (with a fish body burden of 13.9 bq mg⁻¹) or directly exposed to DIC-14 medium at a higher concentration of 1000 bq mL⁻¹. (d) A prediction of the geometry showed that serine substrate combined with PLP aldimine in the SHMT structure (PDB ID codes 4OT8) approached to the His129. (e) KIE_c predicted for the rate-limiting TS as His129-PLP-serine composite for the donation of a methyl to THF. (*) indicates statistically significant differences (*P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001).