Figure 3.

Figure 3.

Blending of C-14 in the metabolism of biomolecules. (a) KEGG global pathway map of identified and annotated metabolites labeled in dietary exposed zebrafish brain by feeding with daphnia containing OBC-14 (red) or OBC-13 (brown). Representative ¹⁴C-labeled amino acids are marked as abbreviation. Inferred potentially active biosynthesis pathways are highlighted. Map generated using iPath tools (version 3) [39]. (b) Distributions of 47 ¹⁴C-labeled metabolites in zebrafish brain in the metabolic pathways. The circle size in the right panel represents the ratio of the number of labeled metabolites to the number of metabolites in a pathway. (c) Measured radioactivity in extracted serine by combined application of HPLC and LSC. In HPLC-LSC practice, a narrow section of eluent exactly at the retention time (RT) of serine in HPLC was flowed into LSC for in-time quantification on radiation signal. Asterisks (*) or NS indicate statistically significant or insignificant differences.