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Fig. 3 Ombuoside protects HEI-OC1 cells against cisplatin-induced reactive oxygen species (ROS) and mitochondrial membrane potential (MMP). A?D. Immunofluorescence detection of apoptotic cells by staining for cleaved caspase 3 (green) and nuclei (blue)by 4?,6-diamidino-2-phenylindole (DAPI), N = 3.E?H. Detection of mitochondrial ROS (mtROS) levels using MitoSOX Red vmtROS (red), N = 3. I?L. Measurement of intracellular total ROS (green) using a CellROX Green probe, N = 3. M ? P Measurement of the MMP using MitoTracker® Red probe (red), N = 3. Scale bar: 50 ?m. Q-T. Quantification of positive cell in Fig. .3A?P. The percentage value was obtained from the ratio of positive cell number to total cell number in the same view field at the same magnification by manually counting. Data are presented as mean ± SEM. ????P ? 0.0001.