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Fig. 4 The influence of T3 exposure on the expression of select genes and type I collagen in cultured trout cardiac fibroblasts. Panels A-D) Relative expression of col1a1, mmp2, timp2 and mmp9, respectively, normalized to β-actin. E) The expression levels of type I collagen normalized to total protein in cardiac fibroblasts. Fibroblasts were exposed to 0 nM, 10 nM or 70 nM T3. F) Representative image of western blot stained for type I collagen. N = 3–4 cell lines, each individual cell line is composed of the mean of 4 cell passages. IC = internal control.
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Reprinted from Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 299, Long, R.R.B., Bullingham, O.M.N., Baylis, B., Shaftoe, J.B., Dutcher, J.R., Gillis, T.E., The influence of triiodothyronine on the immune response and extracellular matrix remodeling during zebrafish heart regeneration, 111769, Copyright (2024) with permission from Elsevier. Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.