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Fig. 9 1D 1H STD NMR spectra for the compound 24e recorded at an Hsp90β:ligand ratio of 1:200 and 600 MHz. The molecular structure illustrates the proton nomenclature and the color-coded relative degrees of saturation of the individual not-overlapping protons. The STD amplification factors were normalized to the intensity of the signal with the largest STD effect. Reference STD spectra (top) with proton assignment and difference STD spectra (bottom) are shown. The unassigned proton signals between 3.5 and 3.8 ppm belong to the protein buffer with glycerol. The proton signals were calibrated to the DSS signal at 0.0 ppm. The spectra are not to scale.