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Fig. 6 Measurement of glutathione content in zebrafish embryo hearts using monochlorobimane. Embryos subjected to hypoxia and treated for 24 h with DMSO, 50 µM empagliflozin (empa 50) or 40 µM MMP13 inhibitor (MMP13 I) were stained with monochlorobimane (mBCI) and imaged with fluorescence microscope. A) Reflected white light and fluorescence images of mBCI in zebrafish embryos. B) Quantitation of integrated mBCI fluorescence signal intensity in the heart region. normoxia, n=14; hypoxia, n=12; empa 50 + hypoxia, n=11; MMP13 I + hypoxia, n=13. Scale bars 100 µm. **P<0.01, *P<0.05. Capped bars on top of the columns indicate standard error of the means.