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Fig. 5 Measurement of apoptosis in zebrafish embryo hearts using acridine orange. Embryos subjected to hypoxia and treated for 6 h with DMSO, empagliflozin (empa 50, 50 µM) or MMP13 inhibitor (MMP13 I, 40 µM) were stained with acridine orange (AO) and imaged with fluorescence microscope. A) Reflected white light (RL) and fluorescence images (AO) of zebrafish embryos. B) Quantitation of mean AO fluorescence intensity in the heart region. normoxia, n=11; hypoxia, n=9; empa 50 + hypoxia, n=7; MMP13 I + hypoxia, n=6. Scale bars 100 µm. **P<0.01, *P<0.05. Capped bars on top of the columns indicate standard error of the means.