FIGURE 5

FIGURE 5

Preventing cellular senescence by inactivating p16 rescues trabecular bone loss in Gorab^Prx1 mutants. (a) 3D rendering, (b) representative microCT images and quantitation of (c) percentage of trabecular bone volume (BV/TV); (d) trabecular number (Tb.N); (e) trabecular separation (Tb.Sp); and (f) trabecular thickness (Tb.Th) at the proximal tibia of female control, Gorab^Prx1 and Gorab^Prx1;Cdkn2a^Null mice at 12 weeks of age (N = 8). Scanned and analyzed with Bruker Skyscan 1172 at 5 μm. Scale bar = 500 μm. Histomorphometric analysis at the proximal tibia secondary spongiosa in control, Gorab^Prx1 and Gorab^Prx1;Cdkn2a^Null mice for (g) number of osteoblast per bone perimeter (N.Ob/B.Pm) and (h) number of osteoclast per bone perimeter (N.Oc/B.Pm) (N = 5). *p < 0.05, **p < 0.01, ***p < 0.001, ns, no statistically significant difference (unpaired Welch's t‐test).