ZFIN Image: Kim et al., 2025, Figure 7

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Figure 7

Regulation of stemness, EMT and angiogenesis by downregulating AQP3. (A) Expression of AQP3 and cancer stem cell markers in AQP3-knockdown H1299 cells. (B) Sphere-forming ability in AQP3-knockdown H1299 cells. Scale bar, 100 μm. (C) Expression of AQP3 and EMT markers in AQP3-knockdown H1299 cells. (D) Migratory and invasive ability of AQP3-knockdown H1299 cells. Scale bar, 200 μm. (E) Angiogenesis activity suppressed by conditioned medium (fresh EBM media/H1299 cultured media with siAQP3 ratios (75:25, 50:50). (F) VEGF expression. ^**P<0.01, ^***P<0.001. EMT, epithelial-mesenchymal transition; AQP, aquaporin; RT, reverse transcription; WB, western blotting; si, small interfering; OCT, octamer binding transcription factor; KLF, Kruppel-like factor; VEGF, vascular endothelial growth factor; RCF, relative colony forming; ZEB, Zinc-finger E-box-binding homeobox.

Acknowledgments

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