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Fig. 2 A. Impact of empagliflozin on zebrafish embryos’ hearts under hypoxic exposure. Fractional shortening (FS) of the beating hearts of zebrafish embryos was measured in normoxia and hypoxia, as well as in hypoxia after treatment with empagliflozin (50 and 100 µM). **** P<0.0001, *** P<0.001, **P<0.01, *P<0.05. Capped bars on top of the columns indicate standard error of the means.B. Relative expression of Bnp determined with RT-qPCR of hypoxia-exposed zebrafish embryos treated with empagliflozin (50 µM or 100 µM). Bnp expression was downregulated in response to empagliflozin treatment. *** P<0.001, **P<0.01, *P<0.05. Capped bars on top of the columns indicate standard error of the means.