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Fig. 8 Mitochondria ultrastructural changes in 7 dpf dldh–/– larvae fast-twitch skeletal muscle fibers. (A and B) Mitochondrial ultrastructure was grossly disrupted in 7 dpf dldh–/– fibers, as compared with WT. Scale bars: 500 nm. (C) Percentage of damaged mitochondria in WT and dldh–/– fibers. (D–F) Mitochondrial ultrastructure morphological features of roundness, circularity, and solidity were quantitatively analyzed using ImageJ. (G and H) Mitochondria number relative to fiber cross-sectional area (μm–2) and average mitochondrial area per fiber cross-sectional area were analyzed using imageJ. In total, 110 (WT) and 102 (dldh–/–) in 24 (WT) and 15 (dldh–/–) fast-twitch muscle fibers from 3 WT and 3 dldh–/– larvae were analyzed. ****P < 0.0001, *P < 0.05 by unpaired Student’s t test. Data are shown as mean ± SD.