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Fig. 6
WNK1 was expressed in the endothelium of human atherosclerotic plaques. Endothelial WNK1 expression was determined by immunofluorescent staining of sections of human carotid artery plaques (symptomatic (N = 5); asymptomatic (N = 3) with anti-WNK1 (red) and anti-VWF antibodies (green) to identify EC. Nuclei were counterstained using DAPI (blue). Staining was analysed by fluorescence microscopy and the proportion of WNK1-positive endothelial cells calculated.Representative images are shown. WNK1—positive EC are indicated with arrows. Differences between means were calculated using an unpaired t-test.
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