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Fig. 5
WNK1 is enriched at a low WSS region of the mouse endothelium. WNK1 was analysed by en face staining of the murine aorta using anti-WNK1 antibodies and Alexafluor568-conjugated secondary antibodies (red) (n = 3 mice). EC were co-stained using anti-CDH5 (green) and nuclei were detected with TO-PRO-3 (blue). WNK1 expression was quantified in high and low WSS regions. Mean values + /− standard error of mean are shown. Differences between means were analysed using a paired t-test.
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