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Fig. 2 Disrupting Hsd17b12a results in organ expansion defects.a?c The development of the endocrine pancreas, exocrine pancreas, and liver was examined using WISH (whole-mount in situ hybridization) at 3 dpf, 4 dpf, and 5 dpf in both WT and hsd17b12a?/? (n > 3). Scale bar, 200 ?m. d?f The areas of ?-cells (ins), exocrine pancreas (trypsin), and liver (fabp10a) were measured in WT and hsd17b12a?/? embryos at 3 dpf, 4 dpf and 5 dpf. g Fluorescence images of exocrine pancreas and liver in WT, hsd17b12a?/?, and Tg (CMV: hsd17b12a)/hsd17b12a?/? at 5 dpf (n > 3). Scale bar, 200 ?m. h The areas of images of the liver and exocrine pancreas were quantified in WT, hsd17b12a?/? and Tg(CMV:hsd17b12a)/hsd17b12a?/?. Data in d?f, h are represented as mean ± S.D., using a two-tailed Student?s t test; each point represents an independent ±biological sample (n > 3). Source data are provided as a Source Data file.