GPN treatment reduces the number of vesicles accumulating G418. (A) Image of neuromast treated with G418-TR. (A') Mask of segmented hair cells. (A”) Mask of segmented vesicles. (B) Image of neuromast treated with G418-TR and GPN. (B') Mask of segmented hair cells. (B”) Mask of segmented vesicles. (C) Number of vesicles per hair cell, mean for each neuromast analyzed. ****Mann-Whitney test, p < 0.0001. (D) Vesicle volume per hair cell, mean for each neuromast. ns, Mann-Whitney test. (E) Vesicle fluorescence per hair cell, mean for each neuromast analyzed. ns, Mann-Whitney test. Error bars represent Standard Deviation.
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