Fig. 7

Fig. 7 Activation of Gck ameliorates hyperglycemia.

a Experimental setup and timeline created in https://BioRender.com. b Glucose measurements of pdx1^-/- after 5 days treatment with either 2 µM Dorzagliatin or 500 µM D-Mannoheptulose reveal a glucose lowering effect of Dorzagliatin (N = 4) as shown in a dot plot with indicated mean. Statistical significance assessed by One-way ANOVA. P-values of multiple comparisons are indicated. c Measurement of Gck activity in pdx1^-/- following treatments as indicated (N > 3) as shown in a dot plot with indicated mean. Statistical significance assessed by One-way ANOVA. P-values of multiple comparisons are indicated. dgck:GFP expression following Dorzagliatin or D-Mannoheptulose treatment in pdx1^-/- larvae. Stress-induced fluorescence signal quantification (e) and representative images (f) showing gck:GFP and oxidative stress as assessed by EpRE:RFP expression in the liver of control DMSO-treated and pdx1^-/- treated with DMSO, Dorzagliatin or D-Mannoheptulose. (Liver is outlined in white). Dot and bar plot showing mean with SD. Statistical significance assessed by One-way ANOVA. P-values of multiple comparisons are indicated. Scale bar: 100 µm.