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Fig. EV5 Sub-TAD looping mediated enhancer-promoter interaction of scar-6/prozb locus. (A) Hi-C heatmap representation of Human SCAR-6 locus in HepG2 at 10 kb resolution from ENCODE database (ENCODE Project Consortium et al, 2020; Wang et al, 2018) and zebrafish scar-6 locus in brain tissue at 5 kb resolution from (Yang et al, 2020). (B) UCSC genome browser snapshot of data for CTCF binding peaks at 24 hpf of zebrafish for at scar-6 and prozb locus (Pérez-Rico et al, 2020). (C) Bar plot representing ChIP-qPCR quantifying fold enrichment using CTCF antibody for scar-6 locus in wild type and scar-6gib007Δ12/Δ12 mutant zebrafish. Data from 3 independent biological replicates plotted as mean fold enrichment ± standard deviation; *P < 0.05, **P < 0.01 (two-tailed unpaired t-test). (D) Schematic and western blot of DNA-pulldown assay was performed using streptavidin tagged scar-6 gene DNA in zebrafish, and immuno blotting was done using prdm14 antibody. N = 3.