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Fig. EV4 CRISPRi of scar-6 locus exhibits haemorrhage phenotype. (A) Representation of CRISPR-dCas9-KRAB mediated inhibition of TF binding on scar-6 locus. (B) Representative image showing fluorescence for blood (gata1a:DsRed) in the cranial region of 3 dpf zebrafish and o-dianisidine stating of RBC blood cells in control and dCas-9-KRAB + sgscar-6 injected zebrafish. The black and white arrowhead denotes hemorrhage in the animals. 4× magnification; scale bar = 200 μm. (C) Box and whisker plot representing the percentage of animals exhibiting haemorrhage phenotype in control and dCas-9-KRAB + sgscar-6 injected zebrafish. Data from 3 independent biological replicates are represented by boxes indicating the interquartile range (25th to 75th percentiles), with the horizontal line within each box denoting the median. Whiskers extend to 1.5 times the interquartile range to define the minimum and maximum values, while individual points represent data from each experiment; *p < 0.05 (two-tailed unpaired t-test).