IMAGE

Fig. EV2

ID
ZDB-IMAGE-241126-33
Source
Figures for Ranjan et al., 2024
Image
Figure Caption

Fig. EV2 CRISPR-Cas9 mediated mutant generation of scar-6 lncRNA gene. (A) DNA-PAGE gel illustrating the results of the heteroduplex mobility assay (HMA) conducted on the scar-6 targeted region in F1 scar-6 mutant adult zebrafish, with genotypes identified by in-dels. Extra bands in the PAGE gel represent heteroduplex templates formed due to heterozygosity in the target region. The red asterisk denotes heterozygous mutant animals. (B) Representative image showing blood vessels of 3 dpf zebrafish progeny derived from wild-type gib004Tg(fli1a:EGFP;gata1a:DsRed) and scar-6gib007Δ12/Δ12 zebrafish. 2.5× magnification; scale bar = 500 μm. (C) Representative image showing secondary structure of scar-6 RNA under wildtype and 12-bp edited condition. The highlighted region represent change in the structure of lncRNA.

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