Fig. 5

Fig. 5 Measurement of ROS and temperature in zebrafish embryos under mitochondrial toxic stress.

a Schematic illustration of the experimental process for measurement, including steps: (i) nanoprobe approaching the embryo, (ii) embryo deformation, (iii) insertion into the perivitelline fluid, (iv) insertion into the embryo. b Corresponding bright-field images of a zebrafish embryo measured at each step in (a). c Current trace corresponding to ROS detection in a zebrafish embryo measured at each step described in (a). d Current traces collected from the nanoprobe after penetrating single zebrafish embryos treated with FCCP and a control group (no FCCP), with an applied potential of 0.85 V. e Comparison of ROS concentration in five zebrafish embryos from the FCCP-treated group and the control group. f Time variation of temperature traces measured in zebrafish embryos after FCCP treatment and in the control group. g Comparison of temperature in five zebrafish embryos from the FCCP-treated group and the control group