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Fig. 3 Evaluation of the sleep-promoting effects of 1?4 in a pentobarbital-induced sleep mouse model. (A) The experimental procedure for the pentobarbital-induced sleep test method. (B?D) The effect of compounds 1?4 on sleep latency (B), sleep duration (C), and blood melatonin concentration (D) in mice treated with pentobarbital. Error bars represent the mean ± SD. Significance was evaluated using a t-test. Significance levels are indicated as *p < 0.05, **p < 0.01, and ***p < 0.005, compared to the control (CONT.).
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