ZFIN Image: Zhao et al., 2024, Figure 5

Image

Figure Caption

Figure 5

Knockdown of glulb induces apoptosis in zebrafish hair cells, and the supplementation of glutamine fails to rescue the observed phenotype. (A) The zebrafish embryos treated with glulb-MO and the control zebrafish were subjected to TUNEL staining, which revealed the presence of apoptotic signals, specifically in the hair cells of the glulb-MO-treated zebrafish. (B) The percentage of hair cells displaying apoptotic signals was evaluated in control zebrafish embryos and glulb-MO-injected zebrafish. TUNEL+/GFP+ denotes hair cells exhibiting apoptotic signals, while TUNEL-/GFP+ represents hair cells lacking apoptotic signals. Chi-squared test: ****, p < 0.0001. (C) The phenotype of hair cell clusters in whole embryonic zebrafish was visualized using confocal laser microscopy. Significantly reduced hair cell clusters were observed in glulb-MO-treated zebrafish compared to Ctrl zebrafish, and co-injection of glulb-MO and glutamine failed to rescue the reduction in hair cell clusters. (D) Similarly, a decreased number of hair cells was observed in the inner ear of zebrafish embryos treated with glulb-MO compared to control zebrafish, and co-injection of glulb-MO and glutamine failed to rescue the phenotype. Lateral crista (LC), anterior crista (AC) and posterior crista (PC). (E) The number of hair cells exhibited a significant reduction statistically, with no substantial rescue observed following glutamine supplementation. One-way ANOVA: ****, p < 0.0001; **, p < 0.01, ns means no significant.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.