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Fig. 8 Effects of s100a1 haploinsufficiency on male zebrafish liver regeneration following PH. (A) IF staining of Pcna and Hnf4? in s100a1+/? and wild-type (WT) male zebrafish livers following PH or sham surgery. (B) Quantification of Pcna and Hnf4? double-positive proliferating hepatocytes based on A. Each symbol represents one zebrafish. (C) LBR changes in s100a1+/? males compared with WT males during PH-induced liver regeneration (n=4). (D) Expression of s100a1, ctgfa, amotl2b and cyr61 in s100a1+/? and WT livers at 6 h or 24 h post-PH as determined by RT-qPCR (n=3). Scale bar: 20 ?m. ns, not significant (P>0.05); *P?0.05, **P?0.01 (two-tailed unpaired Student's t-test).