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Fig. 5 Androgen receptor (AR) activity regulates male zebrafish liver regeneration following PH in a Yap1-dependent manner. (A) Expression of cyp19a1b, sult2st3 and tmprss2 in zebrafish livers of both sexes at 6 h post-PH as determined by RT-qPCR (n=3 for cyp19a1b, n=4 for the remaining groups). (B) Expression of ctgfa and cyr61 in flutamide/DMSO-treated male zebrafish livers at 24 h post-PH/sham surgery as determined by RT-qPCR (n=3). (C) IF staining of Pcna and Hnf4α in DMSO/flutamide-treated male zebrafish livers following PH or sham surgery. (D) Quantification of Pcna and Hnf4α double-positive proliferating hepatocytes based on C. Each symbol represents one zebrafish. (E) Changes in LBR of DMSO/flutamide-pretreated LiPan male zebrafish during PH-induced liver regeneration (n=4). (F) IF staining of Pcna and Hnf4α in dihydrotestosterone (DHT)/DHT+VP/DMSO-pretreated zebrafish livers of both sexes at 24 h post-PH. (G) Quantification of Pcna and Hnf4α double-positive proliferating hepatocytes based on F. Each symbol represents one zebrafish. (H) Expression of ctgfa, amotl2b and cyr61 in DHT/DMSO-pretreated male zebrafish livers at 24 h post-PH as determined by RT-qPCR (n=3). Scale bars: 20 μm. ns, not significant (P>0.05); *P≤0.05, **P≤0.01 (two-tailed unpaired Student's t-test).