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Fig. 6 RNA isolation and qRT-PCR of regeneration-specific markers. (A) 1% (w/v) agarose gel in 1xTBE shows two RNA-specific bands (28 s and 18 s), and control and 7 dpl brain samples are shown in the gel followed by 1 kb ladder. (B) 0.8% (w/v) agarose gel in 1xTBE gel shows primer-specific PCR products upon RT-PCR (Sox2-240bp, olig2-224bp, and gfap-158bp). The control and 7 dpl samples show the correct product size for their respective primers. As expected, NTC (no template control does not give a product). Note: the gel is cut to show only relevant lanes. (C) Analysis of qRT-PCR using delta-delta ct method (rpl13a was used as housekeeping). p-value < 0.05 for sox2 and olig2, p-value <0.01 for gfap.