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Fig. 5 Expression of Paqr5b in olfactory mucosa (OM). (A) Expression analysis of Paqr5b in the OM. Proteins in the OM of paqr5b+/+ and paqr5b?/? zebrafish were detected by Coomassie Brilliant Blue R staining (CBBR). Paqr5b in the OM was detected with an anti-zebrafish Paqr5b antibody (a-Paqr5b). The arrowhead indicates a protein band corresponding to Paqr5b. (B) Immunohistochemical observation of Paqr5b in OM. Immunohistochemical staining results for Paqr5b in paqr5b+/+ and paqr5b?/? OMs. Sagittal sections of the OM of paqr5b+/+ and paqr5b?/? zebrafish were stained with an anti-zebrafish Paqr5b antibody (a-Paqr5b) and DAPI. Images of DAPI signals observed through a DAPI filter (DAPI) and images of immunostaining signals observed through the FITC filter. Merged images (Merge) of differential interference contrast (DIC) images and images of DAPI and anti-Paqr5b (a-Paqr5b) staining are shown. Enlarged images of DAPI and anti-Paqr5b-stained sections are indicated by red squares (lower panels). Neuronal cells (Crypt, Microvillus and Ciliated) that reacted with anti-Paqr5b in paqr5b+/+ are indicated by arrows. These OSNs are absent in paqr5b?/?. Nonspecific staining with the anti-Paqr5b antibody is indicated by a white arrowhead. The scale bars indicate 200 ?m in the upper panels and 50 ?m in the enlarged images. (C) Sagittal sections of paqr5b+/+ and paqr5b?/? were stained with hematoxylin, eosin and Alcian blue (H-E-A). The stained glycan layers are indicated by arrows. Scale bars are 200 ?m in the upper panels and 50 ?m in the lower panels.