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Fig. 3 (A) Tube tunnel escape test after 1-hour inter-trial (memory retention) interval in the 50 cm tube with 1.6 cm diameter. Wild-type (n = 24) and autism model zebrafish (n = 10) escape latency is shown as a function of time (trials). Escape latency in the sixth trial for both groups measured after 1 hour from the last trial. Statistical significance (comparing escape latency at the 6th trial to that at the 1st trial) is shown as *p < 0.05. (B) Escape latencies of wild-type zebrafish (n = 14) trained over 3 days and long-term memory performance tested 4 days later, that is, on day 7 are shown. Statistical significance to evaluate whether escape latency remains reduced on the 7th day compared with the 1st day is shown as *p < 0.05. (C) Escape time for wild-type (n = 30) and autism KO model (n = 10) zebrafish in different colored tubes compared with the transparent tube (control). Mean + S.E.M. are shown. Statistical significance (comparing performance at a given trial to that at the first trial) is indicated as *p < 0.05, **p < 0.01, ***p < 0.001.