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Fig. 4 Compatibility of the RADISH design with multiple wounding techniques. (A) 3 dpf Tg(krt4:Gal4, UAS:GCaMP6f) x (cdh1-dTomato)xt18 embryo wounded via manual tailfin transection. (B) Embryo wounded via laser ablation. (C,D) The same embryos 4 min later, showing the progression of calcium transients after wounding. Scale bars = 100 µm. Abbreviations: RADISH = Rotational Assistant for Danio Imaging of Subsequent Healing; dpf = days post fertilization
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