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Fig. 3 Improved quality of laser scanning confocal images of 3 dpf embryos in the RADISH design. (A) Representative image of 3 dpf Tg(krt4:Gal4, UAS:GCaMP6f) embryo stained with MemGlow 560 without using the RADISH. (B) Representative image of Tg(krt4:Gal4, UAS:GCaMP6f) embryo stained with MemGlow 560 mounted in the RADISH. Scale bar = 100 µm. Abbreviations: RADISH = Rotational Assistant for Danio Imaging of Subsequent Healing; dpf = days post fertilization
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Full text @ J. Vis. Exp.