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Fig. 1 Design and use of PET laminate microfluidics device (RADISH). (A) Schematic illustrating individual PET layers' arrangement and thickness, assembly, and device mounting and use. (B) Final product, mounted in a ¾" cutout in a 35 mm dish. (C) 3 dpf embryos, (D) 5 dpf embryos, (E) 14 dpf embryos housed inside the device. Scale bars = 1,000 µm (C,D,E). Abbreviations: PET = polyethylene terephthalate; RADISH = Rotational Assistant for Danio Imaging of Subsequent Healing; dpf = days post fertilization
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Full text @ J. Vis. Exp.