Figure 15

Figure 15

Behavioral analysis of anxiety by the new tank test of adult male and female zebrafish (three months) with diet-induced obesity (DIO) for eight weeks. Data are expressed as medians (interquartile distance) analyzed using the Kruskal–Wallis test. Each circle represents an individual animal (n = 15). Control group 1: fed with Artemia sp. (first month: 15 mg/day/fish and in the second month 30 mg/day/fish); control group 2: fed with commercial food (3.5% of the group’s average weight); obesity group 1: overfed with powdered egg yolk mixed with soybean oil (2.5:1 w/w)—5% of average body weight; obesity group 2—overfed with Artemia sp. in the first month (60 mg/day/fish) and in the second month (120 mg/day/fish). Asterisks indicate significant statistical differences: * p < 0.05; ** p < 0.01; *** p < 0.0001. (a) Total distance traveled (m); (b): Average speed (m/s); (c) Immobile time (s); (d): Time at the top (s); (e) Top: latency to first entry.