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Figure 7
Synergistic effect of krasV12 expression and LPS/DSS on intestinal tumorigenesis. Four-month-postfertilization wild-type and kras+ zebrafish were cotreated with 2 μM of mifepristone, 40 ng/mL of LPS, and 0.0625% DSS for 4 weeks, and samples were then collected for gross observations and histological analyses. There were four experimental groups: WT, WT/DSS/LPS, kras+, and kras+/DSS/LPS. (A,B) Body length and body weight. (C) Survival curves. (D) Examples of normal intestines, enteritis, hyperplasia, and tubular adenoma as revealed by H&E staining of intestinal sections. (E) Summary of intestinal histological abnormalities observed in the four experimental groups. These data were generated as a result of a blinded histological analysis (WT, N = 10; WT/DSS/LPS, N = 10; kras+, N = 11; kras+ with DSS/LPS, N = 11). Differences among the variables were assessed using Student’s t-tests or one-way ANOVA. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar: 50 μm.