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Figure 6
Cotreatment with DSS and LPS exacerbated the increased number of neutrophils and macrophages in the intestines of kras+/lyz+ and kras+/mepg1+ zebrafish. (A,C) Fluorescence of neutrophils (WT/lyz+, N = 30; WT/lyz+/DSS/LPS, n = 32; kras+/lyz+, n = 31; kras+/lyz+/DSS/LPS, n = 31) or macrophages (WT/mpeg1+, N = 30; WT/mepg1+/DSS/LPS, N = 30; kras+/mpeg1+, N = 30; kras+/mepg1+/DSS/LPS, N = 30) in the intestines. (B,D) Quantification of the number of positive cells as revealed by fluorescence of neutrophils or macrophages. Differences among the variables were assessed using Student’s t-tests. Statistical significance: *** p < 0.001. Scale bar: 25 μm.