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Fig. 8 Exogenous nucleoside supplementation restores neutropenia in dnajc21 mutants.A Lateral views of Sudan Black staining in wildtype and dnajc21−/− mutant embryos at 48 hpf following treatment with 100 mM uridine or 100 mM thymidine from 3 to 48 hpf. Experiments were done in two biological replicates, each comprising at least 20–30 embryos per condition. Numbers on the lower right indicate the number of larvae with the same phenotype. Number of neutrophils per embryo is quantified in the graph. B Brightfield lateral view images of wildtype and dnajc21−/− mutant larvae untreated or treated with 100 mM thymidine from 3 hpf to 4 dpf. Graph shows quantification of larval length. Two biological replicates, each comprising at least 20 embryos per genotype, were analyzed. hpf: hours post-fertilization; dpf: days post-fertilization. *p < 0.01; ***p < 0.0001; ****p < 0.00001.