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Fig. EV1 Effects of mRpL4 RNAi on Notch signal activity A–C. Representative image showing the expression of NRE‐GFP in larval neuroblasts (n > 10 larvae) of control (A), mRpL24 RNAi (B) and mRpL4 RNAi (C) larvae. D–F. Representative image showing the expression of NRE‐GFP in salivary gland imaginal rings (n > 10 larvae) of control (D), mRpL24 RNAi (E) and mRpL4 RNAi (F) larvae. G–I. Representative image showing the expression of Su(H)‐LacZ in midgut cells (n > 10 flies) of control (G) and mRpL4 RNAi (H, I) adult flies. J. The level of Su(H) occupancy at Wg, Cut and Vg genomic regions as assessed by qPCR following ChIP, from wild‐type and UAS‐mRpL4‐RNAi‐expressing wing disks. Data are presented as mean ± SEM, two biological replicates for each genotype and three technical replicates for each sample. Statistical significance was tested using two‐tailed unpaired t‐test. *P < 0.05, **P < 0.01, ns means “not significant”.