Figure 3

Figure 3

Whole-mount in situ hybridization analysis of ankha and ankhb spatial expression patterns in zebrafish development. Lateral views of zebrafish larval stages at 2, 3 and 4 days post-fertilization (dpf) reveal spatial gene expression patterns for ankha and ankhb. At 2 and 3 dpf, both ankha and ankhb show predominant staining in the craniofacial region, somites and notochord. At 4 dpf, ankha extends below the eye (star) and appears in the somites and notochord (arrows). ankhb expression at 3 dpf and 4 dpf is mainly localized to the craniofacial region and notochord area. For ankhb, staining below the eye is reduced and is more localized in regions below the hindbrain. Noto: notochord, Som: somites. The yolk was removed from the embryo; the scale bar indicates 100 μm.