Figure 2

Figure 2

Temporal expression of ankha and ankhb during zebrafish development. (a) Expression of ankha and ankhb relative to the lsm12b control in this representative-endpoint PCR gel, N = 3 replicates. Thirty embryos or fry from each developmental stage, ranging from the 2-cell stage (0.75 hpf) to the 7 dpf stage, were sampled. (b) RT-qPCR normalized expression of ankha and ankhb genes at 2, 3, 4 and 7 days post-fertilization (dpf) relative to gapdh expression, showing the mean +/− the standard error. Each sample represents 30 pooled larvae, where N = 3, and statistical significance was determined using an unpaired t-test (p < 0.05).