Fig. 5 Expression of her5 and her11 in wild-type and mib1 ta52 b mutant embryos at the bud and 24 hpf stages. The expression of her5 (A,B) and her11 (C,D) was detected in wild-type siblings (left panels) and mib1 ta52 b homozygous mutants (right panels) at the bud (A,C) and 24 hpf (B,D) stages. Dorsal views with anterior toward the top. hb, hindbrain; mb, midbrain; mhb, mid-hindbrain boundary. qRT-PCR results show the quantification of expression levels. Quantitative data are presented as mean ± standard error of mean (SEM). Statistical analysis was performed by Student?s t-test. All reactions were performed in triplicate for each sample. *, p < 0.05; ***, p < 0.001.
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