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Fig. 4 Comparison of her8 a, her8.2, her13, and hes6 mRNA expression in wild-type and mib1 ta52 b mutant embryos by in situ hybridization. The expression of her8 a (A,B), her8.2 (C,D), her13 (E,F), and hes6 (G,H) were detected in wild-type siblings (left panels) and mib1 ta52 b homozygous mutant embryos (right panels) at the bud stage (A,C,E,G) and 24 hpf (B,D,F,H). Dorsal views with anterior toward the top. ad, anterior diencephalon; fb, forebrain; hb, hindbrain; in, interneurons; mb, midbrain; mhb, mid-hindbrain boundary; mn, motor neurons; psm, presomitic mesoderm, r, retina; rb, rhombomeres; rob, rhombomere boundaries, tc, telencephalon; sn, sensory neurons; sc, spinal cord. The expression levels were validated using quantitative real-time polymerase chain reaction (qRT-PCR). Quantitative data are presented as mean ± standard error of mean (SEM). Statistical analysis was performed by Student?s t-test. All reactions were performed in triplicate for each sample. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.