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Fig. 7 - supplement 1 Expression and function of CXCR4 and CXCR4 mutants coupled to AcGFP in transfected JK-/- cells. (A) Flow cytometry analysis of cells transiently transfected with the indicated CXCR4 mutants or wt CXCR4, coupled to AcGFP and their expression was determined 24 hours after transfection by GFP detection in flow cytometry. A representative experiment of 4 performed is shown. (B) CXCL12-induced migration of CXCR4-defficient Jurkat cells (JK-/-) transiently transfected with CXCR4wt or its mutants, CXCR4I204K, CXCR4G207I, CXCR4L208K, CXCR4R235L, CXCR4F249L, CXCR4Y256F and CXCR4S260A. Data are shown as the mean ± SD percentage of input cells that migrate (n = 3).