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Fig. 7 C21ORF2-V58L impairs motor behavior in zebrafish embryos. A Representative images of 5 days post-fertilization (5 dpf) zebrafish embryos non-injected and injected with C21ORF2-WT or C21ORF2-V58L constructs. B Quantification of the number of deformed 5 dpf zebrafish embryos as in A. Pooled percentages from five independent experiments (total numbers of embryos analyzed—281 non injected embryos, 77 GFP injected embryos, 113 C21ORF2-WT injected embryos, 82 C21ORF2-V58L embryos). Data are shown as means with SEM *p < 0.05, **p < 0.01 Kruskal–Wallis test, Dunn’s multiple comparison post-hoc. C, D Zebrabox analysis of zebrafish embryo motor behavior at 5 dpf. Time spent swimming (seconds) and trajectory covered (millimeters) during 1 h were quantified. Pooled data from 4 independent experiments are plotted as means with SEM *p < 0.05, **p < 0.01, ****p < 0.0001 Kruskal–Wallis test with Dunn’s multiple comparison post-hoc test. Scale bar: A, 1 mm