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Fig. 1 Expression analysis of bait proteins for interactome analysis. A Schematic representation of GFP-tagged C21ORF2, KIF5A, NEK1, TBK1, TUBA4A and FUS. NTD—N-terminal domain; CTD—C-terminal domain; CCD—coil-coiled domain; KD—kinase domain; BD—basic domain; ULD—ubiquitin-like domain; SDD—scaffold and dimerization domain; QGSY—Glu-Gly-Ser-Tyr-rich region; G rich—Gly-rich region; E—nuclear export signal; RRM—RNA recognition motif; RGG—Arg-Gly-rich region; Z—zinc finger motif; L—nuclear localization signal. B–H Left panel: Expression of GFP and GFP-tagged proteins in Neuro2A (N2a) cells assessed by immunohistochemistry (DAPI in blue). Exogenous proteins show an endogenous distribution pattern. C–H Right panel: Western blots showing GFP-tagged (black arrowhead) and endogenous (white arrowhead) proteins. Anti-β-actin is used as loading control. Scale bar B-H: 5 µm